Mass spectrometry: The components within the sample undergo ionization in the ion source, giving rise to charged ions with varying charge - to - mass ratios. An ion beam is generated via the accelerated electric field, which successively bombards the detector. Subsequently, the corresponding spectrum is collected and computed by the computer.

Agena--MassARRAY system workflow

Technical Advantages and Characteristics of Nucleic Acid Mass Spectrometry

The initial quantity of DNA can be as low as 5 pg, and low-solubility and fragmented templates, such as cell-free DNA (cfDNA) and formalin-fixed paraffin-embedded (FFPE) samples, are applicable.

The integration of single - base extension (SBE) and mass spectrometry technology has notably enhanced the reliability of the test results.

The primers employed do not require special labeling. The operation process occurs on the same plate and well, without intermediate steps of plate transfer and purification, featuring a high degree of automation and a short manual operation duration.